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Article

Focused directed evolution of pentaerythritol tetranitrate reductase by using automated anaerobic kinetic screening of site-saturated libraries.

Chembiochem : a European journal of chemical biology - 22 Nov 2010

Hulley Martyn E, Toogood Helen S, Fryszkowska Anna, Mansell David, Stephens Gill M, Gardiner John M, Scrutton Nigel S

Abstract excerpt

This work describes the development of an automated robotic platform for the rapid screening of enzyme variants generated from directed evolution studies of pentraerythritol tetranitrate (PETN) reductase, a target for industrial biocatalysis. By using a 96-well format, near pure enzyme was recove...

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PMID: 21064170
DOI: 10.1002/cbic.201000527

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-21064170-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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