Article
Expression and function of chicken integrin beta 1 subunit and its cytoplasmic domain mutants in mouse NIH 3T3 cells.
The Journal of cell biology - 1 Jan 1990
Hayashi Y, Haimovich B, Reszka A, Boettiger D, Horwitz A
Abstract excerpt
Chicken integrin beta 1 cDNA and its site-directed mutants were cloned into a mammalian expression vector and introduced into mouse NIH 3T3 cells. Stable transfectants expressing the chicken beta 1 subunit or its site-directed mutants were identified by immunostaining with antibodies specific for...
Topics
- Amino Acid Sequence
- Animals
- Blotting, Northern
- Cell Adhesion
- Cell Membrane
- Cells, Cultured
- Chickens
- Chimera
- Cloning, Molecular
- Cytoplasm
- Cytoskeletal Proteins
- DNA
- Fluorescent Antibody Technique
- Integrins
- Macromolecular Substances
- Mice
- Mice, Inbred Strains
- Molecular Sequence Data