Article
X-ray crystallography, mass spectrometry and single crystal microspectrophotometry: a multidisciplinary characterization of catechol 1,2 dioxygenase.
Biochimica et biophysica acta - 1 Jun 2011
Micalella Chiara, Martignon Sara, Bruno Stefano, Pioselli Barbara, Caglio Raffaella, Valetti Francesca, Pessione Enrica, Giunta Carlo, Rizzi Menico
Abstract excerpt
Intradiol-cleaving catechol 1,2 dioxygenases are Fe(III) dependent enzymes that act on catechol and substituted catechols, including chlorocatechols pollutants, by inserting molecular oxygen in the aromatic ring. Members of this class are the object of intense biochemical investigations aimed at the understanding of their catalytic mechanism, particularly for designing mutants with selected catalytic properties....
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