Article
Molecular characterization of pmbA, an Escherichia coli chromosomal gene required for the production of the antibiotic peptide MccB17.
Molecular microbiology - 1 Nov 1990
Rodríguez-Sáinz M C, Hernández-Chico C, Moreno F
Abstract excerpt
Microcin B17 (MccB17) is a peptide antibiotic produced by Escherichia coli strains harbouring plasmid pMccB17. We have isolated two mutations that strongly reduce the production of MccB17. These mutations, which map at 96 min on the E. coli chromosome, define a new gene that we have called pmbA. A chromosomal DNA fragment of about 13 kb, including the wild-type pmbA allele, was cloned into a mini-Mu plasmid...
Topics
- Amino Acid Sequence
- Anti-Bacterial Agents
- Bacteriocins
- Base Sequence
- Chromosomes, Bacterial
- Cloning, Molecular
- DNA, Bacterial
- Escherichia coli
- Genes, Bacterial
- Genotype
- Molecular Sequence Data