Article
Nucleotide sequence of the Escherichia coli regulatory gene mprA and construction and characterization of mprA-deficient mutants.
Journal of bacteriology - 1 Jun 1991
del Castillo I, González-Pastor J E, San Millán J L, Moreno F
Abstract excerpt
In high copy number, the Escherichia coli mprA gene reduces the synthesis of peptide microcins B17 and C7 (MccB17 and MccC7) and blocks the osmoinduction of the proU operon at the transcriptional level. mprA has been sequenced and shown to encode a polypeptide of 176 amino acids (Mr, 20,563). Insertion and deletion mutant mprA alleles were constructed and then transferred to the chromosome by allelic replacement....
Topics
- Alleles
- Amino Acid Sequence
- Bacteriocins
- Base Sequence
- Escherichia coli
- Genes, Bacterial
- Genes, Regulator
- Molecular Sequence Data
- Mutation
- Operon
- Osmolar Concentration