Article
Characterization of Escherichia coli ATP synthase beta-subunit mutations using a chromosomal deletion strain.
Biochemistry - 16 Jul 1991
Lee R S, Pagan J, Wilke-Mounts S, Senior A E
Abstract excerpt
(1) We constructed Escherichia coli strain JP17 with a deletion in the ATP synthase beta-subunit gene. JP17 is completely deficient in ATP synthase activity and expresses no beta-subunit. Expression of normal beta-subunit from a plasmid restores haploid levels of ATP synthase in membranes. JP17 was shown to be efficacious for studies of beta-subunit mutations. Site-directed mutants were studied directly in JP17....
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