Article
In-gel technology for PCR genotyping and pathogen detection.
Analytical chemistry - 1 Oct 2010
Atrazhev Alexey, Manage Dammika P, Stickel Alexander J, Crabtree H John, Pilarski Linda M, Acker Jason P
Abstract excerpt
This work describes the use of polyacrylamide gel and PCR reagents photopolymerized in a mold to create an array of semisolid posts that serve as reaction vessels for parallel PCR amplification of an externally added template. DNA amplification occurred in a cylindrical, self-standing 9 × 9 array of gel posts each less than 1 μL in volume. Photopolymerization of the gel with an intercalating dye added prior to...
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