Article
Fatty acid modulation and polyamine block of GluK2 kainate receptors analyzed by scanning mutagenesis.
The Journal of general physiology - 1 Sept 2010
Wilding Timothy J, Chen Kevin, Huettner James E
Abstract excerpt
RNA editing of kainate receptor subunits at the Q/R site determines their susceptibility to inhibition by cis-unsaturated fatty acids as well as block by cytoplasmic polyamines. Channels comprised of unedited (Q) subunits are strongly blocked by polyamines, but insensitive to fatty acids, such as arachidonic acid (AA) and docosahexaenoic acid (DHA), whereas homomeric edited (R) channels resist polyamine block but...
Topics
- Alanine
- Amino Acid Sequence
- Arginine
- Cell Line
- Chlorides
- Docosahexaenoic Acids
- Humans
- Ion Channel Gating
- Membrane Potentials
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Patch-Clamp Techniques