Article

A Comprehensive Analysis of Novel Disulfide Bond Introduction Site into the Constant Domain of Human Fab

2021-03-17

Abstract excerpt

Generally, intermolecular disulfide bond contribute to the conformational protein stability. To identify sites where intermolecular disulfide bonds can be introduced into the Fab’s constant domain of the therapeutic IgG, Fab mutants were predicted using the MOE software, a molecular simulator, and expressed in Pichia pastoris. SDS-PAGE analysis of the prepared Fab mutants from P. pastoris indicated that among the...

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Literature Corpus work: 9326fe0a-21ae-5cfe-9452-78c786afe6d7
DOI: 10.21203/rs.3.rs-294111/v1

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