Monitoring of protein conformation by high-performance size-exclusion liquid chromatography and scanning diode array second-derivative UV absorption spectroscopy.

Abstract excerpt

Genetic methods now allow the rapid production of mutant proteins for structure-function analysis. To properly interpret any change in biologic activity resulting from modification in primary sequence, it is essential to monitor conformational changes resulting from mutations. Several methods allow low-resolution protein conformational analysis. One method, second-derivative UV absorption spectroscopy, is...

Topics

• Amino Acid Sequence
• Chromatography, Gel
• Chromatography, High Pressure Liquid
• Interleukin-2
• Molecular Sequence Data
• Mutation
• Protein Conformation
• Spectrophotometry, Ultraviolet