Identification and in vivo analysis of murine hematopoietic stem cells.

doi:10.1016/S0076-6879(10)76023-7

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Article

Identification and in vivo analysis of murine hematopoietic stem cells.

Methods in enzymology - 1 Jan 2010

Avagyan Serine, Amrani Yacine M, Snoeck Hans-Willem

Abstract excerpt

Hematopoietic stem cells (HSCs) can self-renew and give rise to all the cells of the blood and the immune system. As they differentiate, HSCs progressively lose their self-renewal capacity and generate lineage-restricted multipotential progenitor cells that in turn give rise to mature cells. The development of rigorous quantitative in vivo assays for HSC activity combined with multicolor flow cytometry and...

Topics

Animals
Cell Culture Techniques
Cell Differentiation
Cell Lineage
Cell Separation
Flow Cytometry
Hematopoietic Stem Cell Transplantation
Hematopoietic Stem Cells
Mice
Mice, Inbred C57BL
Phenotype

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Open in PubMed

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PMID: 20691879
DOI: 10.1016/S0076-6879(10)76023-7

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