Article
Assessing loss of imprint methylation in sperm from subfertile men using novel methylation polymerase chain reaction Luminex analysis.
Fertility and sterility - 1 Jan 2011
Sato Akiko, Hiura Hitoshi, Okae Hiroaki, Miyauchi Naoko, Abe Yukiko, Utsunomiya Takafumi, Yaegashi Nobuo, Arima Takahiro
Abstract excerpt
OBJECTIVE: To assess the clinical value of bisulfite polymerase chain reaction Luminex (BPL), an automated, high-throughput procedure for the detection of alterations in DNA methylation. DESIGN: Experimental prospective study. SETTING: University research laboratory and private in vitro fertilization (IVF) clinic. PATIENT(S): A total of 337 men, 61 with severe oligozoospermia, 67 with moderate oligozoospermia,...
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