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Article

Melting temperature of surface-tethered DNA.

Analytical biochemistry - 1 Nov 2010

Nasef Hany, Ozalp V Cengiz, Beni Valerio, O'Sullivan Ciara K

Abstract excerpt

A method for the accurate determination of the melting temperature (T(m)) of surface-immobilized DNA duplexes that exploits the fluorescence-quenching properties of gold is reported. A thiolated single-stranded DNA probe is chemisorbed onto a gold surface and then hybridized to a fluorophore-labeled complementary sequence. On formation of the duplex, the fluorescence of the label is effectively quenched by the...

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Identifiers

PMID: 20615383
DOI: 10.1016/j.ab.2010.06.049

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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