Article
Temperature- and flow-enhanced detection specificity of mutated DNA against the wild type with reporter microspheres.
The Analyst - 21 Oct 2013
Kirimli Ceyhun E, Shih Wei-Heng, Shih Wan Y
Abstract excerpt
Detection of mutated (MT) deoxyribonucleic acid (DNA) amongst the wild type (WT) requires the probe DNA (pDNA) that is complementary to the MT to discriminate the WT by one or two nucleotide mismatches. Traditionally this is achieved by raising the temperature to above the melting temperature (Tm) of the WT (TWT) but below that of the MT (TMT). However, a raised temperature is also accompanied by a weakened...
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