Using student-generated UV-induced Escherichia coli mutants in a directed inquiry undergraduate genetics laboratory.

doi:10.1534/genetics.110.120147

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Article

Using student-generated UV-induced Escherichia coli mutants in a directed inquiry undergraduate genetics laboratory.

Genetics - 1 Sept 2010

Healy Frank G, Livingstone Kevin D

Abstract excerpt

We report a thematic sequence of directed inquiry-based labs taking students from bacterial mutagenesis and phenotypic identification of their own self-created mutant, through identification of mutated genes by biochemical testing, to verification of mutant alleles by complementation, and finally to mutant allele characterization by DNA sequence analysis. The lab utilizes UV mutagenesis with wild-type Escherichia...

Topics

Amino Acid Sequence
Base Sequence
Escherichia coli
Genetics
Laboratories
Lactose
Membrane Transport Proteins
Molecular Sequence Data
Mutagenesis
Mutation
Problem-Based Learning
Students
Ultraviolet Rays
Universities

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Identifiers

PMID: 20592259
DOI: 10.1534/genetics.110.120147
PMCID: PMC2940297

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