Article
An undergraduate laboratory class using CRISPR/Cas9 technology to mutate drosophila genes.
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology - 6 May 2016
Adame Vanesa, Chapapas Holly, Cisneros Marilyn, Deaton Carol, Deichmann Sophia, Gadek Chauncey, Lovato TyAnna L, Chechenova Maria B, Guerin Paul, Cripps Richard M
Abstract excerpt
CRISPR/Cas9 genome editing technology is used in the manipulation of genome sequences and gene expression. Because of the ease and rapidity with which genes can be mutated using CRISPR/Cas9, we sought to determine if a single-semester undergraduate class could be successfully taught, wherein stud...
Topics
- Amino Acid Sequence
- Animals
- Base Sequence
- CRISPR-Cas Systems
- Drosophila
- Drosophila Proteins
- Female
- Gene Targeting
- Genetic Engineering
- Genome, Insect
- Male
- Molecular Biology
- Mutation
- Plasmids