Article
Sodium 4-phenylbutyrate ameliorates the effects of cataract-causing mutant gammaD-crystallin in cultured cells.
Molecular vision - 4 Jun 2010
Gong Bo, Zhang Li-Yun, Lam Dennis Shun-Chiu, Pang Chi-Pui, Yam Gary Hin-Fai
Abstract excerpt
PURPOSE: gammaD-Crystallin (CRYGD) is a major structural lens crystallin and its mutations result in congenital cataract formation. In this study, we attempted to correct the altered protein features of G165fsX8 CRYGD protein with small chemical molecules. METHODS: Recombinant FLAG-tagged mutants...
Topics
- Animals
- Apoptosis
- COS Cells
- Cataract
- Chlorocebus aethiops
- Dose-Response Relationship, Drug
- HSP70 Heat-Shock Proteins
- Mutation
- Nuclear Envelope
- Phenotype
- Phenylbutyrates
- Solubility
- Tissue Distribution
- Transfection
- Up-Regulation
- gamma-Crystallins