Article
Tracing antigen signatures in the human IgE repertoire.
Molecular immunology - 1 Aug 2010
Marth Katharina, Novatchkova Maria, Focke-Tejkl Margarete, Jenisch Stefan, Jäger Siegfried, Kabelitz Dieter, Valenta Rudolf
Abstract excerpt
Allergen recognition by IgE antibodies is a key event in allergic inflammation. In this study, the IgE IGHV repertoires of individuals with allergy to the major birch pollen allergen, Bet v 1, were analyzed over a four years period of allergen exposure by RT-PCR and sequencing of cDNA. Approximately half of the IgE transcripts represented non-redundant sequences, which belonged to seventeen different IGHV genes....
Topics
- Allergens
- Antibody Specificity
- Antigens, Plant
- Base Sequence
- Betula
- DNA Primers
- Genes, Immunoglobulin Heavy Chain
- Histocompatibility Testing
- Humans
- Immunoglobulin E
- Immunoglobulin Variable Region
- Mutation
- Pollen
- Radioallergosorbent Test