Article
A flagella-less mutant of Borrelia burgdorferi. Structural, molecular, and in vitro functional characterization.
The Journal of clinical investigation - 1 Jul 1991
Sadziene A, Thomas D D, Bundoc V G, Holt S C, Barbour A G
Abstract excerpt
A nonmotile mutant of Borrelia burgdorferi, the etiologic agent of Lyme disease, was isolated and characterized. The mutant was compared with the wild-type predecessor as well as with a motile back-revertant of the same genetic background. The mutant lacked, by morphologic, biochemical, and immunologic criteria, the major structural protein of flagella, flagellin. This mutation was not associated with major DNA...
Topics
- Blotting, Western
- Borrelia burgdorferi Group
- DNA, Bacterial
- Electrophoresis, Polyacrylamide Gel
- Endothelium, Vascular
- Enzyme-Linked Immunosorbent Assay
- Flagella
- Microscopy, Electron
- Mutation