Article
Casein kinase 2-dependent phosphorylation of human Rad9 mediates the interaction between human Rad9-Hus1-Rad1 complex and TopBP1.
Genes to cells : devoted to molecular & cellular mechanisms - 1 Jun 2010
Takeishi Yukimasa, Ohashi Eiji, Ogawa Kaori, Masai Hisao, Obuse Chikashi, Tsurimoto Toshiki
Abstract excerpt
The checkpoint clamp Rad9-Hus1-Rad1 (9-1-1) is loaded by the Rad17-RFC complex onto chromatin after DNA damage and plays a key role in the ATR-dependent checkpoint activation. Here, we demonstrate that in vitro casein kinase 2 (CK2) specifically interacts with human 9-1-1 and phosphorylates serines 341 and 387 (Ser-341 and Ser-387) in the C-terminal tail of Rad9. Interestingly, phosphorylated Ser-387 has...
Topics
- Carrier Proteins
- Casein Kinase II
- Cell Cycle Proteins
- Cells, Cultured
- DNA-Binding Proteins
- Exonucleases
- HeLa Cells
- Humans
- Mutation
- Nuclear Proteins
- Phosphorylation
- Serine