Mutagenesis of the 3' nontranslated region of Sindbis virus RNA.

Abstract excerpt

A cDNA clone from which infectious RNA can be transcribed was used to construct 42 site-specific mutations in the 3' nontranslated region of the Sindbis virus genome. The majority of these mutations were made in the 3'-terminal 19-nucleotide conserved sequence element and consisted of single nucleotide substitutions or of small (1 to 8) nucleotide deletions. An attempt was made to recover mutant viruses after...

Topics

• Animals
• Base Sequence
• Cloning, Molecular
• Introns
• Molecular Sequence Data
• Mutation
• Nucleotide Mapping
• Plasmids
• RNA, Viral
• Sindbis Virus
• Transfection
• Viral Plaque Assay