Unexpectedly High Copy Number of Random Integration but Low Frequency of Persistent Expression of the <i>Sleeping Beauty</i> Transposase After <i>Trans</i> Delivery in Primary Human T Cells

Abstract excerpt

We have shown that the Sleeping Beauty (SB) transposon system can mediate stable expression of both reporter and therapeutic genes in human primary T cells and that trans delivery (i.e., transposon and transposase are on separate plasmids) is at least 3-fold more efficient than cis delivery. One concern about trans delivery is the potential for integration of the transposase-encoding sequence into the cell genome...

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