Article
The change of immunoactivity of dendritic cells induced by mouse 4-1BBL recombinant adenovirus.
Yonsei medical journal - 1 Jul 2010
Youlin Kuang, Xiaodong Weng, Xiuheng Liu, Zhiyuan Chen, Hengcheng Zhu, Hui Chen, Botao Jiang
Abstract excerpt
PURPOSE: The purpose of this study is to construct a recombinant adenovirus vector carrying mouse 4-1BBL and observe its effects in dendritic cells. MATERIALS AND METHODS: Mouse 4-1BBL cDNA was taken from the plasmid pcDNA3-m4- 1BBL and subcloned into adenovirus shuttle plasmid pAdTrack-CMV, and then transformed into competent BJ5183 with plasmid pAdEasy-1. After recombination in E.coli, Ad-4-1BBL was packaged...
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