Article
Alanine scanning mutagenesis of HIV-1 gp41 heptad repeat 1: insight into the gp120-gp41 interaction.
Biochemistry - 22 Jun 2010
Sen Jayita, Yan Tianran, Wang Jizhen, Rong Lijun, Tao Lin, Caffrey Michael
Abstract excerpt
On the basis of mutagenesis, biochemical, and structural studies, heptad repeat 1 of HIV gp41 (HR1) has been shown to play numerous critical roles in HIV entry, including interacting with gp120 in prefusion states and interacting with gp41 heptad repeat 2 (HR2) in the fusion state. Moreover, HR1 is the site of therapeutic intervention by enfuviritide, a peptide analogue of HR2. In this study, the functional...
Topics
- Alanine
- Amino Acid Sequence
- Amino Acid Substitution
- Cell Line
- HIV Envelope Protein gp120
- HIV Envelope Protein gp41
- HIV-1
- Humans
- Membrane Fusion
- Models, Molecular
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Folding
- Repetitive Sequences, Amino Acid
- Virus Internalization