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A New Strategy to Identify and Annotate Human RPE-Specific Gene Expression

7 May 2010

Abstract excerpt

BACKGROUND: To identify and functionally annotate cell type-specific gene expression in the human retinal pigment epithelium (RPE), a key tissue involved in age-related macular degeneration and retinitis pigmentosa. METHODOLOGY: RPE, photoreceptor and choroidal cells were isolated from selected freshly frozen healthy human donor eyes using laser microdissection. RNA isolation, amplification and hybridization to...

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PMID: 20479888
DOI: 10.1371/journal.pone.0009341

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