Article
Genetic and biochemical analyses of sensor kinase A in Bacillus subtilis sporulation.
Genetics and molecular research : GMR - 30 Mar 2010
Nguele J C, Eswaramoorthy P, Bhattacharya M, Ngou-Milama E, Fujita M
Abstract excerpt
Temporal and spatial regulation of gene expression during endospore formation in Bacillus subtilis prompted us to investigate the molecular mechanisms that coordinate the phosphorelay. We targeted KinA for random mutagenesis. In addition, we constructed KinA-GFP transcriptional fusions for verification, via fluorescence. Four distinct types of sporulation-defective mutants were identified as inactive (no...
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