Article
Accessing carboxylesterase diversity from termite hindgut symbionts through metagenomics.
Journal of molecular microbiology and biotechnology - 1 Jan 2012
Rashamuse Konanani, Mabizela-Mokoena Nobalanda, Sanyika Tendai Walter, Mabvakure Batsirai, Brady Dean
Abstract excerpt
A shotgun metagenomic library was constructed from termite hindgut symbionts and subsequently screened for esterase activities. A total of 68 recombinant clones conferring esterolytic phenotypes were identified, of which the 14 most active were subcloned and sequenced. The nucleotide lengths of the esterase-encoding open reading frames (ORFs) ranged from 783 to 2,592 bp and encoded proteins with predicted...
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