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Article

Identification of a histidyl residue in the active center of endoglucanase D from Clostridium thermocellum.

The Journal of biological chemistry - 5 Jun 1991

Tomme P, Chauvaux S, Béguin P, Millet J, Aubert J P, Claeyssens M

Abstract excerpt

Diethylpyrocarbonate modification of endoglucanase D from Clostridium thermocellum, cloned in Escherichia coli, resulted in a rapid but partial (maximally 70-80%) loss of activity. The second-order rate constant of inactivation proved to be exceptionally high (3210 M-1.min-1). A 3-fold reduction of the kcat and a 2-fold increase of the Km for 2'-chloro-4'-nitrophenyl beta-cellobioside were observed....

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PMID: 2037583

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