Article
Identification of a histidyl residue in the active center of endoglucanase D from Clostridium thermocellum.
The Journal of biological chemistry - 5 Jun 1991
Tomme P, Chauvaux S, Béguin P, Millet J, Aubert J P, Claeyssens M
Abstract excerpt
Diethylpyrocarbonate modification of endoglucanase D from Clostridium thermocellum, cloned in Escherichia coli, resulted in a rapid but partial (maximally 70-80%) loss of activity. The second-order rate constant of inactivation proved to be exceptionally high (3210 M-1.min-1). A 3-fold reduction of the kcat and a 2-fold increase of the Km for 2'-chloro-4'-nitrophenyl beta-cellobioside were observed....
Topics
- Amino Acid Sequence
- Binding Sites
- Blotting, Western
- Cellulase
- Chromatography, High Pressure Liquid
- Clostridium
- Histidine
- Kinetics
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation