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Article

Construction and properties of a triprotein containing the high-affinity nickel transporter of Alcaligenes eutrophus.

FEBS letters - 20 May 1991

Wolfram L, Eitinger T, Friedrich B

Abstract excerpt

The high-affinity nickel transporter of Alcaligenes eutrophus H16 is encoded by gene hoxN, which maps within the hydrogenase gene cluster of megaplasmid pHG1. A tripartite gene fusion was constructed, consisting of (i) the Escherichia coli lacZ gene for beta-galactosidase, (ii) a segment encoding an endoproteolytically cleavable peptide, and (iii) the A. eutrophus gene hoxN. An E. coli strain harboring this...

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Identifiers

PMID: 2037063
DOI: 10.1016/0014-5793(91)80565-k

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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Construction and properties of a triprotein containing the high-affinity nickel transporter of Alcaligenes eutrophus.PMID 2037063Read ↗

Cloning, nucleotide sequence, and heterologous expression of a high-affinity nickel transport gene from Alcaligenes eutrophus.Semantic proximity Read ↗

hyp gene products in Alcaligenes eutrophus are part of a hydrogenase-maturation system.Semantic proximity Read ↗

Analysis of a pleiotropic gene region involved in formation of catalytically active hydrogenases in Alcaligenes eutrophus H16.Semantic proximity Read ↗

hoxX (hypX) is a functional member of the Alcaligenes eutrophus hyp gene cluster.Semantic proximity Read ↗

Subforms and in vitro reconstitution of the NAD-reducing hydrogenase of Alcaligenes eutrophus.Semantic proximity Read ↗

The hydrogenase gene cluster of Rhizobium leguminosarum bv. viciae contains an additional gene (hypX), which encodes a protein with sequence similarity to the N10-formyltetrahydrofolate-dependent enzyme family and is required for nickel-dependent hydrogenase processing and activity.Semantic proximity Read ↗

Duplication of hyp genes involved in maturation of [NiFe] hydrogenases in Alcaligenes eutrophus H16.Semantic proximity Read ↗

Amino acid replacements at the H2-activating site of the NAD-reducing hydrogenase from Alcaligenes eutrophus.Semantic proximity Read ↗

Substrate specificity of nickel/cobalt permeases: insights from mutants altered in transmembrane domains I and II.Semantic proximity Read ↗

Temperature tolerance of hydrogenase expression in Alcaligenes eutrophus is conferred by a single amino acid exchange in the transcriptional activator HoxA.Semantic proximity Read ↗

Identification of a locus within the hydrogenase gene cluster involved in intracellular nickel metabolism in Bradyrhizobium japonicum.Semantic proximity Read ↗

Rhizobium leguminosarum HupE is a highly-specific diffusion facilitator for nickel uptake.Semantic proximity Read ↗

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