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Article

Construction and properties of a triprotein containing the high-affinity nickel transporter of Alcaligenes eutrophus.

FEBS letters - 20 May 1991

Wolfram L, Eitinger T, Friedrich B

Abstract excerpt

The high-affinity nickel transporter of Alcaligenes eutrophus H16 is encoded by gene hoxN, which maps within the hydrogenase gene cluster of megaplasmid pHG1. A tripartite gene fusion was constructed, consisting of (i) the Escherichia coli lacZ gene for beta-galactosidase, (ii) a segment encoding an endoproteolytically cleavable peptide, and (iii) the A. eutrophus gene hoxN. An E. coli strain harboring this...

Topics

Alcaligenes
Amino Acid Sequence
Bacterial Proteins
Base Sequence
Biological Transport
Electrophoresis, Polyacrylamide Gel
Escherichia coli
Gene Expression Regulation, Bacterial
Genes, Bacterial
Molecular Sequence Data

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Nickel

Plasmids

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Identifiers

PMID: 2037063
DOI: 10.1016/0014-5793(91)80565-k

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2037063-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Construction and properties of a triprotein containing the high-affinity nickel transporter of Alcaligenes eutrophus.PMID 2037063Read ↗

Cloning, nucleotide sequence, and heterologous expression of a high-affinity nickel transport gene from Alcaligenes eutrophus.Semantic proximity Read ↗

hyp gene products in Alcaligenes eutrophus are part of a hydrogenase-maturation system.Semantic proximity Read ↗

Analysis of a pleiotropic gene region involved in formation of catalytically active hydrogenases in Alcaligenes eutrophus H16.Semantic proximity Read ↗

hoxX (hypX) is a functional member of the Alcaligenes eutrophus hyp gene cluster.Semantic proximity Read ↗

Subforms and in vitro reconstitution of the NAD-reducing hydrogenase of Alcaligenes eutrophus.Semantic proximity Read ↗

The hydrogenase gene cluster of Rhizobium leguminosarum bv. viciae contains an additional gene (hypX), which encodes a protein with sequence similarity to the N10-formyltetrahydrofolate-dependent enzyme family and is required for nickel-dependent hydrogenase processing and activity.Semantic proximity Read ↗

Duplication of hyp genes involved in maturation of [NiFe] hydrogenases in Alcaligenes eutrophus H16.Semantic proximity Read ↗

Amino acid replacements at the H2-activating site of the NAD-reducing hydrogenase from Alcaligenes eutrophus.Semantic proximity Read ↗

Substrate specificity of nickel/cobalt permeases: insights from mutants altered in transmembrane domains I and II.Semantic proximity Read ↗

Temperature tolerance of hydrogenase expression in Alcaligenes eutrophus is conferred by a single amino acid exchange in the transcriptional activator HoxA.Semantic proximity Read ↗

Identification of a locus within the hydrogenase gene cluster involved in intracellular nickel metabolism in Bradyrhizobium japonicum.Semantic proximity Read ↗

Rhizobium leguminosarum HupE is a highly-specific diffusion facilitator for nickel uptake.Semantic proximity Read ↗

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