Identification of a locus within the hydrogenase gene cluster involved in intracellular nickel metabolism in Bradyrhizobium japonicum.

doi:10.1128/aem.57.12.3502-3510.1991

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Article

Identification of a locus within the hydrogenase gene cluster involved in intracellular nickel metabolism in Bradyrhizobium japonicum.

Applied and environmental microbiology - 1 Dec 1991

Fu C L, Maier R J

Abstract excerpt

A 0.6-kb fragment of DNA involved in intracellular Ni metabolism was isolated and cloned from a cosmid containing 23.2 kb of hydrogenase-related genes of Bradyrhizobium japonicum. This locus is located 8.3 kb upstream of the hydrogenase structural genes. The hydrogenase activity of a mutant with...

Topics

Blotting, Southern
Cloning, Molecular
Conjugation, Genetic
Genes, Bacterial
Hydrogenase
Kinetics
Multigene Family
Mutation
Nickel
Plasmids
Promoter Regions, Genetic
Restriction Mapping
Rhizobiaceae

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