Cloning, nucleotide sequence, and heterologous expression of a high-affinity nickel transport gene from Alcaligenes eutrophus.

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Article

Cloning, nucleotide sequence, and heterologous expression of a high-affinity nickel transport gene from Alcaligenes eutrophus.

The Journal of biological chemistry - 15 Feb 1991

Eitinger T, Friedrich B

Abstract excerpt

High-affinity nickel transport in Alcaligenes eutrophus H16 is mediated by a function designated hoxN. hoxN lies within the hydrogenase gene cluster of megaplasmid pHG1. An insertional mutation at the hoxN locus led to an increased nickel requirement. In this mutant (strain HF260) both autotrophic growth on hydrogen and wild-type level of urease, a nickel-containing enzyme, were dependent on high concentration of...

Topics

Alcaligenes
Alkaline Phosphatase
Amino Acid Sequence
Base Sequence
Biological Transport
Blotting, Western
Cloning, Molecular
DNA Transposable Elements
Electrophoresis, Polyacrylamide Gel
Gene Expression Regulation, Bacterial

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Genes, Bacterial

Molecular Sequence Data

Mutation

Nickel

Open Reading Frames

Plasmids

Promoter Regions, Genetic

Source

Open in PubMed

Identifiers

PMID: 1847142

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1847142-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Cloning, nucleotide sequence, and heterologous expression of a high-affinity nickel transport gene from Alcaligenes eutrophus.PMID 1847142Read ↗

Construction and properties of a triprotein containing the high-affinity nickel transporter of Alcaligenes eutrophus.Semantic proximity Read ↗

hyp gene products in Alcaligenes eutrophus are part of a hydrogenase-maturation system.Semantic proximity Read ↗

hoxX (hypX) is a functional member of the Alcaligenes eutrophus hyp gene cluster.Semantic proximity Read ↗

Analysis of a pleiotropic gene region involved in formation of catalytically active hydrogenases in Alcaligenes eutrophus H16.Semantic proximity Read ↗

Subforms and in vitro reconstitution of the NAD-reducing hydrogenase of Alcaligenes eutrophus.Semantic proximity Read ↗

The hydrogenase gene cluster of Rhizobium leguminosarum bv. viciae contains an additional gene (hypX), which encodes a protein with sequence similarity to the N10-formyltetrahydrofolate-dependent enzyme family and is required for nickel-dependent hydrogenase processing and activity.Semantic proximity Read ↗

Identification of a locus within the hydrogenase gene cluster involved in intracellular nickel metabolism in Bradyrhizobium japonicum.Semantic proximity Read ↗

Duplication of hyp genes involved in maturation of [NiFe] hydrogenases in Alcaligenes eutrophus H16.Semantic proximity Read ↗

Amino acid replacements at the H2-activating site of the NAD-reducing hydrogenase from Alcaligenes eutrophus.Semantic proximity Read ↗

Temperature tolerance of hydrogenase expression in Alcaligenes eutrophus is conferred by a single amino acid exchange in the transcriptional activator HoxA.Semantic proximity Read ↗

Substrate specificity of nickel/cobalt permeases: insights from mutants altered in transmembrane domains I and II.Semantic proximity Read ↗

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