Temperature tolerance of hydrogenase expression in Alcaligenes eutrophus is conferred by a single amino acid exchange in the transcriptional activator HoxA.

doi:10.1128/jb.177.9.2373-2380.1995

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Temperature tolerance of hydrogenase expression in Alcaligenes eutrophus is conferred by a single amino acid exchange in the transcriptional activator HoxA.

Journal of bacteriology - 1 May 1995

Zimmer D, Schwartz E, Tran-Betcke A, Gewinner P, Friedrich B

Abstract excerpt

Expression of the soluble (SH) and membrane-bound (MBH) hydrogenases in the facultatively lithoautotrophic bacterium Alcaligenes eutrophus is dependent on the transcriptional activator HoxA and the alternative sigma factor sigma 54. Deletion analysis revealed that a region 170 bp upstream of the transcriptional start of the SH operon is necessary for high-level promoter activity. Mobility shift assays with DNA...

Topics

Alcaligenes
Amino Acid Sequence
Bacterial Proteins
Base Sequence
DNA, Bacterial
Escherichia coli
Genes, Bacterial
Genes, Regulator
Homeodomain Proteins
Hot Temperature
Hydrogen

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Hydrogenase

Integration Host Factors

Molecular Sequence Data

Mutation

Operon

Promoter Regions, Genetic

Protein Binding

Source

Open in PubMed Open full text

Identifiers

PMID: 7730267
DOI: 10.1128/jb.177.9.2373-2380.1995
PMCID: PMC176894

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7730267-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Temperature tolerance of hydrogenase expression in Alcaligenes eutrophus is conferred by a single amino acid exchange in the transcriptional activator HoxA.PMID 7730267Read ↗

hoxX (hypX) is a functional member of the Alcaligenes eutrophus hyp gene cluster.Semantic proximity Read ↗

hyp gene products in Alcaligenes eutrophus are part of a hydrogenase-maturation system.Semantic proximity Read ↗

Analysis of a pleiotropic gene region involved in formation of catalytically active hydrogenases in Alcaligenes eutrophus H16.Semantic proximity Read ↗

Cloning, nucleotide sequence, and heterologous expression of a high-affinity nickel transport gene from Alcaligenes eutrophus.Semantic proximity Read ↗

Construction and properties of a triprotein containing the high-affinity nickel transporter of Alcaligenes eutrophus.Semantic proximity Read ↗

Functional analysis by site-directed mutagenesis of the NAD(+)-reducing hydrogenase from Ralstonia eutropha.Semantic proximity Read ↗

The AbrB2 autorepressor, expressed from an atypical promoter, represses the hydrogenase operon to regulate hydrogen production in Synechocystis strain PCC6803.Semantic proximity Read ↗

Amino acid replacements at the H2-activating site of the NAD-reducing hydrogenase from Alcaligenes eutrophus.Semantic proximity Read ↗

Subforms and in vitro reconstitution of the NAD-reducing hydrogenase of Alcaligenes eutrophus.Semantic proximity Read ↗

Genes involved in Sec-independent membrane targeting of hydrogenase in Azotobacter chroococcum.Semantic proximity Read ↗

Functional and structural role of the cytochrome b subunit of the membrane-bound hydrogenase complex of Alcaligenes eutrophus H16.Semantic proximity Read ↗

Duplication of hyp genes involved in maturation of [NiFe] hydrogenases in Alcaligenes eutrophus H16.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.