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Article

Single nucleotide polymorphism detection by optical DNA-based sensing coupled with whole genomic amplification.

Analytical and bioanalytical chemistry - 1 Jan 2013

Ermini M L, Mariani S, Scarano S, Campa D, Barale R, Minunni M

Abstract excerpt

The work presented here deals with the optimization of a strategy for detection of single nucleotide polymorphisms based on surface plasmon resonance imaging. First, a sandwich-like assay was designed, and oligonucleotide sequences were computationally selected in order to study optimized conditions for the detection of the rs1045642 single nucleotide polymorphism in the gene ABCB1. Then the strategy was...

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PMID: 22955671
DOI: 10.1007/s00216-012-6345-4

Genes

ABCB1

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