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Article

Enhanced Tn10 and mini-Tn10 precise excision in DNA replication mutants of Escherichia coli K12.

Mutation research - 31 May 2000

Nagel R, Chan A

Abstract excerpt

The precise excision of transposon Tn10 and a mini-Tn10 derivative, inserted in the gal or lac operons, was studied in dnaB252 and dnaE486 temperature-sensitive mutants of Escherichia coli. dnaB codes for a DNA replication helicase and dnaE for the alpha subunit of DNA polymerase III. Mutations in these genes were found to enhance, at the permissive temperature, the precise excision of both genetic elements. The...

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PMID: 10844241
DOI: 10.1016/s0921-8777(00)00008-2

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