Precise excision of Tn10 in Salmonella typhimurium: effects of mutations in the polA, dam, mutH and mutB genes and of methionine or ethionine in the plating medium.

Abstract excerpt

Precise excision of Tn10 occurs at significantly elevated frequencies in cultures of the polA7 mutant strain of Salmonella typhimurium, is further increased in polA7 dam-1 and polA7 mutB strains and decreased in a polA7 mutH background. The numbers of precise excision events occurring in polA7 strains are also significantly increased when methionine (20 micrograms/ml or less) is present in the medium but...

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