Article
Mapping of deletions in the genome of the highly attenuated vaccinia virus MVA and their influence on virulence.
The Journal of general virology - 1 May 1991
Meyer H, Sutter G, Mayr A
Abstract excerpt
Different passages of the vaccinia virus strain Ankara (CVA wild-type) during attenuation to MVA (modified vaccinia virus Ankara) have been analysed to detect alterations in the genome. Physical maps for the restriction enzymes HindIII and XhoI have been established. Six major deletions relative to the wild-type strain CVA could be localized. They reduce the size of the entire genome from 208 kb (CVA wild-type)...
Topics
- Animals
- Cell Line
- Chromosome Deletion
- DNA, Viral
- Electrophoresis, Agar Gel
- Genes, Viral
- Genetic Markers
- HeLa Cells
- Humans
- Mice
- Phenotype
- Restriction Mapping