Article
Disruption of lactate dehydrogenase and alcohol dehydrogenase for increased hydrogen production and its effect on metabolic flux in Enterobacter aerogenes.
Bioresource technology - 1 Oct 2015
Zhao Hongxin, Lu Yuan, Wang Liyan, Zhang Chong, Yang Cheng, Xing Xinhui
Abstract excerpt
Hydrogen production by Enterobacter aerogenes from glucose was enhanced by deleting the targeted ldhA and adh genes responsible for two NADH-consuming pathways which consume most NADH generated from glycolysis. Compared with the wild-type, the hydrogen yield of IAM1183-ΔldhA increased 1.5 fold. Metabolic flux analysis showed both IAM1183-ΔldhA and IAM1183-Δadh exhibited significant changes in flux, including...
Topics
- Alcohol Dehydrogenase
- Bacterial Proteins
- Biofuels
- Bioreactors
- Biotechnology
- Conjugation, Genetic
- Enterobacter aerogenes
- Fermentation
- Glycolysis
- Hydrogen
- L-Lactate Dehydrogenase
- Mutation