Article
Mutations in conserved yeast DNA primase domains impair DNA replication in vivo.
Proceedings of the National Academy of Sciences of the United States of America - 1 May 1991
Francesconi S, Longhese M P, Piseri A, Santocanale C, Lucchini G, Plevani P
Abstract excerpt
To assess the role of eukaryotic DNA primase in vivo, we have produced conditional and lethal point mutations by random in vitro mutagenesis of the PR11 and PR12 genes, which encode the small and large subunits of yeast DNA primase. We replaced the wild-type copies of PRI1 and PRI2 with two pri1 and two pri2 conditional alleles. When shifted to the restrictive temperature, these strains showed altered DNA...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Centrifugation, Density Gradient
- DNA Mutational Analysis
- DNA Primase
- DNA Replication
- DNA, Fungal
- Genes, Fungal
- Molecular Sequence Data