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Article

1H-15N NMR studies of Escherichia coli tRNA(Phe) from hisT mutants: a structural role for pseudouridine.

Biochemistry - 30 Apr 1991

Davis D R, Poulter C D

Abstract excerpt

Escherichia coli tRNA(Phe)U39 was isolated from a specially constructed bacterial strain (DD1003/pRK3) carrying mutations in the hisT gene (the structural gene for tRNA pseudouridine synthase I) and in the pyrB gene (uracil auxotrophy). The pheU gene for tRNA(Phe) under control of the native tRNA promoter was on a multicopy plasmid and gave up to 40-fold overproduction of tRNA(Phe)U39. The double mutant permitted...

Topics

Base Sequence
Chromatography, High Pressure Liquid
Escherichia coli
Magnetic Resonance Spectroscopy
Molecular Sequence Data
Mutation
Nitrogen Isotopes
Nucleic Acid Conformation
Plasmids
Pseudouridine
RNA, Transfer, Phe

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Identifiers

PMID: 2021615
DOI: 10.1021/bi00231a017

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2021615-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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