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Article

The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity.

RNA (New York, N.Y.) - 1 Jul 1998

Motorin Y, Keith G, Simon C, Foiret D, Simos G, Hurt E, Grosjean H

Abstract excerpt

We have previously shown that the yeast gene PUS1 codes for a tRNA:pseudouridine synthase and that recombinant Pus1p catalyzes, in an intron-dependent way, the formation of psi34 and psi36 in the anticodon loop of the yeast minor tRNA(Ile) in vitro (Simos G et al., 1996, EMBO J 15:2270-2284). Usi...

Topics

Cloning, Molecular
Fungal Proteins
Hydro-Lyases
Mutation
Pseudouridine
RNA Precursors
RNA Processing, Post-Transcriptional
RNA, Fungal
RNA, Plant
RNA, Transfer
Recombinant Proteins
Saccharomyces cerevisiae

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Subcellular Fractions

Substrate Specificity

Source

Open in PubMed Open full text

Identifiers

PMID: 9671058
DOI: 10.1017/s1355838298980396
PMCID: PMC1369665

Genes

PUS1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9671058-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity.PMID 9671058Read ↗

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Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae.Semantic proximity Read ↗

Schizosaccharomyces pombe pus1 mutants are temperature sensitive due to decay of tRNAIle(UAU) by the 5'-3' exonuclease Dhp1, primarily targeting the unspliced pre-tRNA.Semantic proximity Read ↗

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Elements essential for accumulation and function of small nucleolar RNAs directing site-specific pseudouridylation of ribosomal RNAs.Semantic proximity Read ↗

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