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Article

The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity.

RNA (New York, N.Y.) - 1 Jul 1998

Motorin Y, Keith G, Simon C, Foiret D, Simos G, Hurt E, Grosjean H

Abstract excerpt

We have previously shown that the yeast gene PUS1 codes for a tRNA:pseudouridine synthase and that recombinant Pus1p catalyzes, in an intron-dependent way, the formation of psi34 and psi36 in the anticodon loop of the yeast minor tRNA(Ile) in vitro (Simos G et al., 1996, EMBO J 15:2270-2284). Using a set of T7 transcripts of different tRNA genes, we now demonstrate that yeast pseudouridine synthase 1 catalyzes in...

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Identifiers

PMID: 9671058
DOI: 10.1017/s1355838298980396
PMCID: PMC1369665

Genes

PUS1

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

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The yeast tRNA:pseudouridine synthase Pus1p displays a multisite substrate specificity.PMID 9671058Read ↗

Pus1p-dependent tRNA pseudouridinylation becomes essential when tRNA biogenesis is compromised in yeast.Semantic proximity Read ↗

Cloning and characterization of the Schizosaccharomyces pombe tRNA:pseudouridine synthase Pus1p.Semantic proximity Read ↗

Pseudouridylation at position 32 of mitochondrial and cytoplasmic tRNAs requires two distinct enzymes in Saccharomyces cerevisiae.Semantic proximity Read ↗

Schizosaccharomyces pombe pus1 mutants are temperature sensitive due to decay of tRNAIle(UAU) by the 5'-3' exonuclease Dhp1, primarily targeting the unspliced pre-tRNA.Semantic proximity Read ↗

Caenorhabditis elegans pseudouridine synthase 1 activity in vivo: tRNA is a substrate, but not U2 small nuclear RNA.Semantic proximity Read ↗

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