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Article

A flow cytometry-based FRET assay to identify and analyse protein-protein interactions in living cells.

PloS one - 22 Feb 2010

Banning Carina, Votteler Jörg, Hoffmann Dirk, Koppensteiner Herwig, Warmer Martin, Reimer Rudolph, Kirchhoff Frank, Schubert Ulrich, Hauber Joachim, Schindler Michael

Abstract excerpt

BACKGROUND: Försters resonance energy transfer (FRET) microscopy is widely used for the analysis of protein interactions in intact cells. However, FRET microscopy is technically challenging and does not allow assessing interactions in large cell numbers. To overcome these limitations we developed a flow cytometry-based FRET assay and analysed interactions of human and simian immunodeficiency virus (HIV and SIV)...

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Identifiers

PMID: 20179761
DOI: 10.1371/journal.pone.0009344
PMCID: PMC2825263

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