Article
P-element insertion alleles of essential genes on the third chromosome of Drosophila melanogaster: correlation of physical and cytogenetic maps in chromosomal region 86E-87F.
Genetics - 1 Dec 1997
Deák P, Omar M M, Saunders R D, Pál M, Komonyi O, Szidonya J, Maróy P, Zhang Y, Ashburner M, Benos P, Savakis C, Siden-Kiamos I, Louis C, Bolshakov V N, Kafatos F C, Madueno E, Modolell J, Glover D M
Abstract excerpt
We have established a collection of 2460 lethal or semi-lethal mutant lines using a procedure thought to insert single P elements into vital genes on the third chromosome of Drosophila melanogaster. More than 1200 randomly selected lines were examined by in situ hybridization and 90% found to contain single insertions at sites that mark 89% of all lettered subdivisions of the Bridges' map. A set of chromosomal...
Topics
- Alleles
- Animals
- Base Sequence
- Chromosome Mapping
- DNA Transposable Elements
- DNA, Complementary
- Drosophila melanogaster
- Female
- Genes, Insect
- Genes, Lethal