Article
Site-directed mutagenesis of Asp853 in Pdr3p transcriptional activator from Saccharomyces cerevisiae.
Yeast (Chichester, England) - 1 May 2010
Dzugasova Vladimira, Borecka Silvia, Batova Monika, Pilisiova Ruzena, Hervayova Nora, Subik Julius
Abstract excerpt
The PDR3 gene encodes one of the main transcriptional activators involved in the control of multidrug resistance in the yeast Saccharomyces cerevisiae. Recently, it has been demonstrated that a specific D853Y mutation results in the loss of transactivation activity of Pdr3p and its conversion to multicopy suppressor of multidrug resistance. In this study, the Asp853 in Pdr3p was replaced by eight different amino...
Read the complete abstract on PubMedTopics
- ATP-Binding Cassette Transporters
- Alleles
- Amino Acid Substitution
- Antifungal Agents
- Cycloheximide
- DNA-Binding Proteins