Article
Identification of the five hydrophilic residues (Lys-217, Lys-218, Arg-359, His-360, and Arg-513) essential for the structure and activity of vitamin K-dependent carboxylase.
Biochemical and biophysical research communications - 9 Oct 1998
Shimizu A, Sugiura I, Matsushita T, Kojima T, Hirai M, Saito H
Abstract excerpt
Vitamin K-dependent carboxylase catalyzes the posttranslational conversion of glutamic acid to gamma-carboxyglutamic acid in vitamin K-dependent proteins. The clustered charged-to-alanine scanning mutagenesis of bovine carboxylase has identified five distinct candidate regions (I. Sugiura et al.,...
Topics
- Alanine
- Amino Acids, Essential
- Animals
- Arginine
- CHO Cells
- Carbon-Carbon Ligases
- Cattle
- Cricetinae
- Cross-Linking Reagents
- Enzyme Activation
- Epoxy Compounds
- Factor IX
- Histidine
- Lysine
- Mutation
- Protein Precursors
- Recombinant Proteins
- Structure-Activity Relationship