Article
A novel approach for isolation and mapping of intron mutations in a ribonucleotide reductase encoding gene (nrdB) of bacteriophage T4 using the white halo plaque phenotype.
Biochemical and biophysical research communications - 29 Oct 1993
Lal S K, Hall D H
Abstract excerpt
The nrdB gene of bacteriophage T4 codes for the small subunit of ribonucleotide reductase and contains a 598 base pair self splicing intron which is closely related to other group I introns of T4 and eukaryotes. The screening, isolation and mapping of the nrbB intron mutations was conducted by th...
Topics
- Bacteriophage T4
- Base Sequence
- Conserved Sequence
- DNA, Viral
- Escherichia coli
- Genes, Viral
- Hydroxylamine
- Hydroxylamines
- Introns
- Mutagenesis
- Phenotype
- Restriction Mapping
- Ribonucleotide Reductases
- Sequence Deletion
- Viral Plaque Assay