Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

PCR with quenching probes enables the rapid detection and identification of ganciclovir-resistance-causing U69 gene mutations in human herpesvirus 6.

Molecular and cellular probes - 1 Aug 2010

Isegawa Yuji, Matsumoto Chisa, Nishinaka Kazuko, Nakano Kazushi, Tanaka Tatsuya, Sugimoto Nakaba, Ohshima Atsushi

Abstract excerpt

A single-nucleotide polymorphism detection assay using PCR with quenching probes (QP-PCR) was developed for the rapid detection of antiviral drug-resistance mutations of human herpesvirus 6 (HHV-6). The mutations examined were in the HHV-6 U69 gene, and were single-base mutations in sequences kno...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA Probes
Share to Ganciclovir
Share to Genes, Viral

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Herpesvirus 6, Human

Share to Mutant Proteins

Share to Nucleic Acid Denaturation

Share to Nucleotides

Share to Transition Temperature

Source

Identifiers

PMID: 20083192
DOI: 10.1016/j.mcp.2010.01.002

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-20083192-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

PCR with quenching probes enables the rapid detection and identification of ganciclovir-resistance-causing U69 gene mutations in human herpesvirus 6.PMID 20083192Read ↗

Selection of the same mutation in the U69 protein kinase gene of human herpesvirus-6 after prolonged exposure to ganciclovir in vitro and in vivo.Semantic proximity Read ↗

Quantitative, Fluorogenic Probe PCR Assay for Detection of Human Herpesvirus 8 DNA in Clinical SpecimensSemantic proximity Read ↗

Novel method based on "en passant" mutagenesis coupled with a gaussia luciferase reporter assay for studying the combined effects of human cytomegalovirus mutations.Semantic proximity Read ↗

Detection of Two Drug-Resistance Mutants of the Cytomegalovirus by High-Resolution Melting Analysis.Semantic proximity Read ↗

Real-Time Quantitative PCR for Human Herpesvirus 6 DNASemantic proximity Read ↗

Genotyping cytomegalovirus UL97 mutations by high-resolution melting analysis with unlabeled probe.Semantic proximity Read ↗

Amino acid changes within conserved region III of the herpes simplex virus and human cytomegalovirus DNA polymerases confer resistance to 4-oxo-dihydroquinolines, a novel class of herpesvirus antiviral agents.Semantic proximity Read ↗

Mapping ganciclovir resistance in the human herpesvirus-6 U69 protein kinase.Semantic proximity Read ↗

Use of lyophilized standards for the calibration of a newly developed real time PCR assay for human herpes type six (HHV6) variants A and B.Semantic proximity Read ↗

Unlabeled probes for the detection and typing of herpes simplex virus.Semantic proximity Read ↗

Solid- and solution-phase synthesis and application of R6G dual-labeled oligonucleotide probes.Semantic proximity Read ↗

Different mutations in the HHV-6 DNA polymerase gene accounting for resistance to foscarnet.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.