Article
Reconstructed evolutionary adaptive paths give polymerases accepting reversible terminators for sequencing and SNP detection.
Proceedings of the National Academy of Sciences of the United States of America - 2 Feb 2010
Chen Fei, Gaucher Eric A, Leal Nicole A, Hutter Daniel, Havemann Stephanie A, Govindarajan Sridhar, Ortlund Eric A, Benner Steven A
Abstract excerpt
Any system, natural or human-made, is better understood if we analyze both its history and its structure. Here we combine structural analyses with a "Reconstructed Evolutionary Adaptive Path" (REAP) analysis that used the evolutionary and functional history of DNA polymerases to replace amino acids to enable polymerases to accept a new class of triphosphate substrates, those having their 3'-OH ends blocked as a...
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