Article
Nucleosome fractionation by mercury affinity chromatography. Contrasting distribution of transcriptionally active DNA sequences and acetylated histones in nucleosome fractions of wild-type yeast cells and cells expressing a histone H3 gene altered to encode a cysteine 110 residue.
The Journal of biological chemistry - 5 Apr 1991
Chen T A, Smith M M, Le S Y, Sternglanz R, Allfrey V G
Abstract excerpt
A technique for the separation of transcriptionally active and inactive nucleosomes by mercury affinity chromatography has been applied to study the nucleosomal distribution of DNA sequences from the GAL1, ACT1, HIS4, MAT alpha, and HMRa genes of yeast. In mammalian cells, the method has been shown to separate active from inactive nucleosomes and to fractionate the active nucleosomes into two classes, one...
Topics
- Acetylation
- Base Sequence
- Chromatography, Affinity
- Cysteine
- DNA, Fungal
- Electrophoresis, Polyacrylamide Gel
- Genes, Fungal
- Histones
- Mercury
- Molecular Sequence Data