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Article

recA gene dependence of replication of the Escherichia coli chromosome initiated by plasmid pUC13 integrated at predetermined sites.

Molecular & general genetics : MGG - 1 Feb 1991

Mao Y M, Shi Q, Li Q G, Sheng Z J

Abstract excerpt

Plasmid pUC13 was used to clone DNA fragments of known sites from the chromosome of Escherichia coli. Each chimeric plasmid was introduced individually into the same dnaA46 mutant strain LC381 and suppressive integration (Sin) strains were selected. By means of cotransduction the null mutation re...

Topics

Chromosome Mapping
Chromosomes, Bacterial
Cloning, Molecular
DNA Replication
Escherichia coli
Genes, Bacterial
Mutation
Plasmids
Rec A Recombinases
Transduction, Genetic

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 2005865
DOI: 10.1007/BF00269854

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-2005865-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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