Article
recA gene dependence of replication of the Escherichia coli chromosome initiated by plasmid pUC13 integrated at predetermined sites.
Molecular & general genetics : MGG - 1 Feb 1991
Mao Y M, Shi Q, Li Q G, Sheng Z J
Abstract excerpt
Plasmid pUC13 was used to clone DNA fragments of known sites from the chromosome of Escherichia coli. Each chimeric plasmid was introduced individually into the same dnaA46 mutant strain LC381 and suppressive integration (Sin) strains were selected. By means of cotransduction the null mutation recA56 was then introduced into each Sin strain and growth of each recA56 derivative at 42 degrees C was scored. Strains...
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