Article
Ultrafast dynamics of resonance energy transfer in myoglobin: probing local conformation fluctuations.
The journal of physical chemistry. B - 28 Jan 2010
Stevens Jeffrey A, Link Justin J, Kao Ya-Ting, Zang Chen, Wang Lijuan, Zhong Dongping
Abstract excerpt
We report here our systematic characterization of resonance energy transfer between intrinsic tryptophan and the prosthetic heme group in myoglobin in order to develop a novel energy-transfer pair as a molecular ruler in heme proteins to study local conformation fluctuations. With site-directed mutagenesis, we designed four tryptophan mutants along the A-helix of myoglobin and each mutant contains only a single...
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